Journal: Clinical and Experimental Pharmacology & Physiology

Article Title: Mechanisms of IL‐17A Neutralisation in Alleviating Renal Fibrosis and Inflammation in Spontaneously Hypertensive Rats

doi: 10.1111/1440-1681.70116

Figure Lengend Snippet: IL‐17A NAb inhibited EMT in SHR renal tissues. (A) Representative IHC images showing the expression of E‐cadherin, α‐SMA, and Collagen III, with quantitative analysis of their positive areas. (B) Representative immunoblots and relative expression levels of E‐cadherin, α‐SMA, and Collagen III proteins. (C) mRNA expression levels of E‐cadherin, α‐SMA, and Collagen III. Data are expressed as mean ± SD ( n = 6).

Article Snippet: Sections were then incubated overnight at 4°C with primary antibodies against: E‐cadherin (Boster, China), Collagen III (Boster, China), inducible nitric oxide synthase (iNOS) (Boster, China), CD86 (Boster, China), arginase‐1 (Arg‐1) (Boster, China), CD163 (Boster, China), α‐smooth muscle actin (α‐SMA) (Cell Signalling Technology, USA).

Techniques: Expressing, Western Blot

Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Pedigrees of the Six Families with TNHP and Sanger Sequencing Confirming the Identified Mutations in TRPV6 that Show Complete Cosegregation with Phenotype

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques: Sequencing

Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Pre- and Postnatal Clinical Findings in Six Subjects with TRPV6 Variants

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques:

Journal: American Journal of Human Genetics

Article Title: TRPV6 Variants Interfere with Maternal-Fetal Calcium Transport through the Placenta and Cause Transient Neonatal Hyperparathyroidism

doi: 10.1016/j.ajhg.2018.04.006

Figure Lengend Snippet: Functional Analysis of TRPV6 Variants

Article Snippet: The membrane was incubated overnight at 4°C with 3% BSA in PBS with 0.1% Tween-20 (PBST) for blocking and then incubated with mouse anti-c- myc antibody (Sigma #M5546, 1/500 dilution in 3% BSA in PBST) or rabbit anti-TRPV6 antibody (Alomone #ACC-036, 1/500) for 2 hr at room temperature.

Techniques: Functional Assay